English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Scanning STED-FCS reveals spatiotemporal heterogeneity of lipid interaction in the plasma membrane of living cells.

MPS-Authors
/persons/resource/persons40292

Honigmann,  A.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons40296

Müller,  V.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons79571

Ta,  H.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons15789

Schönle,  A.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons15210

Hell,  S. W.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons15024

Eggeling,  C.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

Fulltext (public)

2075686.pdf
(Publisher version), 2MB

Supplementary Material (public)

2075686_Suppl.pdf
(Supplementary material), 928KB

Citation

Honigmann, A., Müller, V., Ta, H., Schönle, A., Sezgin, E., Hell, S. W., et al. (2014). Scanning STED-FCS reveals spatiotemporal heterogeneity of lipid interaction in the plasma membrane of living cells. Nature Communications, 5: 5412. doi:10.1038/ncomms6412.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0024-435B-3
Abstract
The interaction of lipids and proteins plays an important role in plasma membrane bioactivity, and much can be learned from their diffusion characteristics. Here we present the combination of super-resolution STED microscopy with scanning fluorescence correlation spectroscopy (scanning STED-FCS, sSTED-FCS) to characterize the spatial and temporal heterogeneity of lipid interactions. sSTED-FCS reveals transient molecular interaction hotspots for a fluorescent sphingolipid analogue. The interaction sites are smaller than 80nm in diameter and lipids are transiently trapped for several milliseconds in these areas. In comparison, newly developed fluorescent phospholipid and cholesterol analogues with improved phase-partitioning properties show more homogenous diffusion, independent of the preference for liquid-ordered or disordered membrane environments. Our results do not support the presence of nanodomains based on lipid-phase separation in the basal membrane of our cultured nonstimulated cells, and show that alternative interactions are responsible for the strong local trapping of our sphingolipid analogue.