Mini-Tn7 vectors for studying post-transcriptional gene expression in 
Pseudomonas

Liu, Yunhao; Rainey, Paul B.; Zhang, Xue-Xian

doi:10.1016/j.mimet.2014.10.015

アイテム詳細

登録内容を編集ファイル形式で保存

一時保存へ追加

リリース履歴を表示詳細要約

公開

学術論文

Mini-Tn7 vectors for studying post-transcriptional gene expression in Pseudomonas

MPS-Authors

/persons/resource/persons56872

Rainey, Paul B.
External Scientific Member Group Experimental and Evolutionary Genetics, Max Planck Institute for Evolutionary Biology, Max Planck Society;

External Resource

There are no locators available

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

フルテキスト (公開)

公開されているフルテキストはありません

付随資料 (公開)

There is no public supplementary material available

引用

Liu, Y., Rainey, P. B., & Zhang, X.-X. (2014). Mini-Tn7 vectors for studying post-transcriptional gene expression in Pseudomonas. Journal of Microbiological Methods, 107, 182-185. doi:10.1016/j.mimet.2014.10.015.

引用: https://hdl.handle.net/11858/00-001M-0000-0024-498F-0

要旨

We describe the construction and validation of five mini-Tn7 vectors for analysis of post-transcriptional gene expression in Pseudomonas. Four vectors allowconstruction of translational fusions to β-galactosidase (lacZ),while the fifth is designed for functional analysis of noncoding RNA genes. Translational fusions can be constructed without a functional promoter in the vector or from an inducible promoter of either Ptac or PdctA. We show that promoterless fusions have value for determining levels of translation, whereas fusions to inducible promoters have utility in the analysis of mRNA-binding factors