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Journal Article

13C-Detection in RNA Bases:  Revealing Structure−Chemical Shift Relationships

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Farès,  Christophe
Department of NMR Based Structural Biology, MPI for Biophysical Chemistry, Max Planck Society;

Amata,  Irene
Department of NMR Based Structural Biology, MPI for Biophysical Chemistry, Max Planck Society;

Carlomagno,  Teresa
Department of NMR Based Structural Biology, MPI for Biophysical Chemistry, Max Planck Society;

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Citation

Farès, C., Amata, I., & Carlomagno, T. (2007). 13C-Detection in RNA Bases:  Revealing Structure−Chemical Shift Relationships. Journal of the American Chemical Society, 129(51), 15814-15823. doi:10.1021/ja0727417.


Cite as: https://hdl.handle.net/11858/00-001M-0000-0024-4B3B-7
Abstract
The chemical shifts of the unprotonated carbons in the proton-deficient nucleobases of RNA are rarely reported, despite the valuable information that they contain about base-pairing and base-stacking. We have developed 13 C-detected 2D-experiments to identify the unprotonated 13C in the RNA bases and have assigned all the base nuclei of uniformly 13C,15N-labeled HIV-2 TAR-RNA. The 13C chemical shift distributions revealed perturbations correlated with the base-pairing and base-stacking properties of all four base-types. From this work, we conclude that the information contained in the chemical shift perturbations within the base rings can provide valuable restraint information for solving RNA structures, especially in conformational averaged regions, where NOE-based information is not available.