Ca2+ triggers massive exocytosis in Chinese hamster ovary cells

Coorssen, J. R.; Schmitt, H. R.; Almers, Wolfhard

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Ca2+ triggers massive exocytosis in Chinese hamster ovary cells

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Almers, Wolfhard
Department of Molecular Cell Research, Max Planck Institute for Medical Research, Max Planck Society;

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Citation

Coorssen, J. R., Schmitt, H. R., & Almers, W. (1996). Ca2+ triggers massive exocytosis in Chinese hamster ovary cells. The EMBO Journal, 15(15), 3787-3791. Retrieved from http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid%3D452059.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0024-515B-3

Abstract

We have tracked the cell surface area of CHO cells by measuring the membrane capacitance, Cm. An increase in cytosolic [Ca2+], [Ca2+]i, increased the cell surface area by 20−30%. At micromolar [Ca2+]i the increase occurred in minutes, while at 20 microM or higher [Ca2+]i it occurred in seconds and was transient. GTPgammaS caused a 3% increase even at 0.1 microM [Ca2+]i. We conclude that CHO cells, previously thought capable only of constitutive exocytosis, can perform Ca2+−triggered exocytosis that is both massive and rapid. Ca2+−triggered exocytosis was also observed in 3T3 fibroblasts. Our findings add evidence to the view that Ca induces exocytosis in cells other than known secretory cells