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Differential Effects of Mutations on the Transport Properties of the Na+/H+ Antiporter NhaA from Escherichia coli

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Mager,  Thomas
Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;

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Braner,  Markus
Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;

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Kubsch,  Bastian
Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;

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Hatahet,  Lina
Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;

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Fendler,  Klaus
Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Mager, T., Braner, M., Kubsch, B., Hatahet, L., Alkoby, D., Rimon, A., et al. (2013). Differential Effects of Mutations on the Transport Properties of the Na+/H+ Antiporter NhaA from Escherichia coli. The Journal of Biological Chemistry, 288(34), 24666-24675.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0024-D4B1-2
Abstract
Na+/H+ antiporters show a marked pH dependence, which is important for their physiological function in eukaryotic and prokaryotic cells. In NhaA, the Escherichia coli Na+/H+ antiporter, specific single site mutations modulating the pH profile of the transporter have been described in the past. To clarify the mechanism by which these mutations influence the pH dependence of NhaA, the substrate dependence of the kinetics of selected NhaA variants was electrophysiologically investigated and analyzed with a kinetic model. It is shown that the mutations affect NhaA activity in quite different ways by changing the properties of the binding site or the dynamics of the transporter. In the first case, pK and/or KDNa are altered, and in the second case, the rate constants of the conformational transition between the inside and the outside open conformation are modified. It is shown that residues as far apart as 15–20 Å from the binding site can have a significant impact on the dynamics of the conformational transitions or on the binding properties of NhaA. The implications of these results for the pH regulation mechanism of NhaA are discussed.