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Journal Article

Robust Electrophysiological Assays using Solid Supported Membranes: the Organic Cation Transporter OCT2

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Gaiko,  Olga
Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;

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Janausch,  Ingo
Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;

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Fendler,  Klaus
Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Gaiko, O., Janausch, I., Geibel, S., Vollert, H., Arndt, P., Gonski, S., et al. (2011). Robust Electrophysiological Assays using Solid Supported Membranes: the Organic Cation Transporter OCT2. Australian Journal of Chemistry, 64(1), 31-35. doi:10.1071/CH10322.


Cite as: https://hdl.handle.net/11858/00-001M-0000-0024-D61F-D
Abstract
An electrophysiological assay platform based on solid supported membranes (SSM) for the organic cation transporter (OCT) is presented. Stable Chinese hamster ovary (CHO) cell lines overexpressing the human (hOCT2) and rat transporters (rOCT2) were generated and validated. Membrane preparations from the cell lines were investigated using SSM-based electrophysiology. Baculovirus transfected insect cells (HighFive and Mimic Sf9) were also tested with the same assay but yielded less than optimal results. The assays were validated by the determination of substrate affinities and inhibition by standard inhibitors. The study demonstrates the suitability of the SSM-based electrophysiological OCT assay for rapid and automatic screening of drug candidates.