Proton transfer in the photosynthetic reaction center of Blastochloris viridis

Kozlova, Maria A.; Juhnke, Hanno; Cherepanov, Dmitry A.; Lancaster, C. Roy D.; Mulkidjanian, Armen Y.

doi:10.1016/j.febslet.2007.12.010

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Proton transfer in the photosynthetic reaction center of Blastochloris viridis

MPS-Authors

/persons/resource/persons137726

Juhnke, Hanno
Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max Planck Society;

/persons/resource/persons137768

Lancaster, C. Roy D.
Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max Planck Society;

External Resource

No external resources are shared

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Kozlova, M. A., Juhnke, H., Cherepanov, D. A., Lancaster, C. R. D., & Mulkidjanian, A. Y. (2008). Proton transfer in the photosynthetic reaction center of Blastochloris viridis. FEBS Letters, 582(2), 238-242. doi:10.1016/j.febslet.2007.12.010.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0024-D82B-1

Abstract

Photosynthetic reaction centers of Blastochloris viridis require two quanta of light to catalyse a two-step reduction of their secondary ubiquinone Q_B to ubiquinol. We employed capacitive potentiometry to follow the voltage changes that were caused by the accompanying transmembrane proton displacements. At pH 7.5 and 20 °C, the Q_B-related voltage generation after the first flash was contributed by a fast, temperature-independent component with a time constant of ∼30 μs and a slower component of ∼200 μs with activation energy (Ea) of 50 kJ/mol. The kinetics after the second flash featured temperature-independent components of 5 μs and 200 μs followed by a component of 600 μs with E_a ∼ 60 kJ/mol.