Three-Dimensional Structure of the Respiratory Chain Supercomplex I1III2IV1 
from Bovine Heart Mitochondria.

Schäfer, Eva; Dencher, Norbert A.; Vonck, Janet; Parcej, David

doi:10.1021/bi700983h

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Three-Dimensional Structure of the Respiratory Chain Supercomplex I₁III₂IV₁ from Bovine Heart Mitochondria.

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Vonck, Janet
Department of Structural Biology, Max Planck Institute of Biophysics, Max Planck Society;

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Parcej, David
Department of Structural Biology, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Schäfer, E., Dencher, N. A., Vonck, J., & Parcej, D. (2007). Three-Dimensional Structure of the Respiratory Chain Supercomplex I₁III₂IV₁ from Bovine Heart Mitochondria. Biochemistry, 46(44), 12579-12585. doi:10.1021/bi700983h.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0024-D8B3-B

Abstract

The respiratory chain complexes can arrange into multienzyme assemblies, so-called supercomplexes. We present the first 3D map of a respiratory chain supercomplex. It was determined by random conical tilt electron microscopy analysis of a bovine supercomplex consisting of complex I, dimeric complex III, and complex IV (I₁III₂IV₁). Within this ₃D map the positions and orientations of all the individual complexes in the supercomplex were determined unambiguously. Furthermore, the ubiquinone and cytochrome c binding sites of each complex in the supercomplex could be located. The mobile electron carrier binding site of each complex was found to be in proximity to the binding site of the succeeding complex in the respiratory chain. This provides structural evidence for direct substrate channeling in the supercomplex assembly with short diffusion distances for the mobile electron carriers.