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A tridecameric c ring of the adenosine triphosphate (ATP) synthase from the thermoalkaliphilic Bacillus sp. strain TA2.A1 facilitates ATP synthesis at low electrochemical proton potential

MPG-Autoren
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Meier,  Thomas
Department of Structural Biology, Max Planck Institute of Biophysics, Max Planck Society;

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Matthies,  Doreen
Department of Structural Biology, Max Planck Institute of Biophysics, Max Planck Society;

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Pogoryelov,  Denys
Department of Structural Biology, Max Planck Institute of Biophysics, Max Planck Society;

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Zitation

Meier, T., Morgner, N., Matthies, D., Pogoryelov, D., Keis, S., Cook, G. M., et al. (2007). A tridecameric c ring of the adenosine triphosphate (ATP) synthase from the thermoalkaliphilic Bacillus sp. strain TA2.A1 facilitates ATP synthesis at low electrochemical proton potential. Molecular Microbiology, 65(5), 1181-1192. doi:10.1111/j.1365-2958.2007.05857.x.


Zitierlink: https://hdl.handle.net/11858/00-001M-0000-0024-D8BF-4
Zusammenfassung
Despite the thermodynamic problem imposed on alkaliphilic bacteria of synthesizing adenosine triphosphate (ATP) against a large inverted pH gradient and consequently a low electrochemical proton potential, these bacteria still utilize a proton-coupled F1Fo-ATP synthase to synthesize ATP. One potential solution to this apparent thermodynamic problem would be the operation of a larger oligomeric c ring, which would raise the ion to ATP ratio, thus facilitating the conversion of a low electrochemical potential into a significant phosphorylation potential. To address this hypothesis, we have purified the oligomeric c ring from the thermoalkaliphilic bacterium Bacillus sp. strain TA2.A1 and determined the number of c-subunits using a novel mass spectrometry method, termed ‘laser-induced liquid bead ion desorption’ (LILBID). This technique allows the mass determination of non-covalently assembled, detergent-solubilized membrane protein complexes, and hence enables an accurate determination of c ring stoichiometries. We show that the Bacillus sp. strain TA2.A1 ATP synthase harbours a tridecameric c ring. The operation of a c ring with 13 subunits renders the thermodynamic problem of ATP synthesis at alkaline pH less severe and may represent a strategy for ATP synthesis at low electrochemical potential.