Amino acid type selective isotope labelling of the multidrug ABC transporter 
LmrA for solid-state NMR studies

Mason, A. James; Siarheyeva, Alena; Haase, Winfried; Lorch, Mark; van Veen, Hendrik; Glaubitz, Clemens

doi:10.1016/j.febslet.2004.05.016

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Amino acid type selective isotope labelling of the multidrug ABC transporter LmrA for solid-state NMR studies

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Haase, Winfried
Department of Structural Biology, Max Planck Institute of Biophysics, Max Planck Society;
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Mason, A. J., Siarheyeva, A., Haase, W., Lorch, M., van Veen, H., & Glaubitz, C. (2004). Amino acid type selective isotope labelling of the multidrug ABC transporter LmrA for solid-state NMR studies. FEBS Letters, 568, 117-121. doi:10.1016/j.febslet.2004.05.016.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0024-DB14-6

Abstract

The ABC transporter LmrA in Lactococcus lactis confers resistance to a wide range of antibiotics and cytotoxic drugs and is a functional homologue of P-glycoprotein. Recently, solid-state NMR methods have shown potential for structural- and non-perturbing, site directed functional studies. These experiments require isotopic labelling of selected sites. We have developed a strategy to produce large quantities of selectively labelled LmrA reconstituted at a high density in lipid membranes. This makes the 64 kDa integral membrane protein LmrA and therefore the ABC transporter superfamily accessible to NMR analysis.