Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
Zur Ablage hinzufügen
  Lokale TagsFreigabegeschichteDetailsÜbersicht

Freigegeben
Buchkapitel

Two-dimensional Crystallization of Membrane Proteins: a Practical Guide

MPG-Autoren
/persons/resource/persons137764

Kühlbrandt,  Werner       
Department of Structural Biology, Max Planck Institute of Biophysics, Max Planck Society;

Externe Ressourcen
Es sind keine externen Ressourcen hinterlegt
Volltexte (beschränkter Zugriff)
Für Ihren IP-Bereich sind aktuell keine Volltexte freigegeben.
Volltexte (frei zugänglich)
Es sind keine frei zugänglichen Volltexte in PuRe verfügbar
Ergänzendes Material (frei zugänglich)
Es sind keine frei zugänglichen Ergänzenden Materialien verfügbar
Zitation

Kühlbrandt, W. (2003). Two-dimensional Crystallization of Membrane Proteins: a Practical Guide. In C. Hunte, G. von Jagow, & H. Schägger (Eds.), Membrane Protein Purification and Crystallization: a Practical Guide. (pp. 253-284). San Diego: Academic Press. doi:10.1016/B978-012361776-7/50016-4.


Zitierlink: https://hdl.handle.net/11858/00-001M-0000-0024-DB2F-C
Zusammenfassung
I. INTRODUCTION

Membrane proteins still present a major challenge in structural biology. Electron microscopy (EM) of two-dimensional (2D) crystals is an increasingly important technique for studying their structure and function. An earlier review (Kühlbrandt, 1992) published about 10 years ago outlines the basic techniques and concepts. While these are unchanged and valid, the number of membrane proteins crystallized in 2D has risen encouragingly in the past three or four years, no doubt largely due to the recent progress in expressing membrane proteins in homologous or heterologous systems. The evaluation of more than 60 2D crystallization protocols now provides a sound base for some general guidelines. Recent technical developments are also discussed in this chapter and may help to facilitate the future production of 2D crystals. Several other reviews published in the past 10 years (Jap et al., 1992; Mosser, 2001; Rigaud et al., 2000; Walz and Grigorieff, 1998) address various aspects of 2D crystallisation.