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Charge displacements during ATP-hydrolysis and synthesis of the Na+ -transporting FoF1-ATPase of Ilyobacter tartaricus

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Burzik,  Christiane
Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;

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Bamberg,  Ernst
Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;
Goethe-Universität Frankfurt, 60439 Frankfurt am Main, Germany;

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Fendler,  Klaus
Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Burzik, C., Kaim, G., Dimroth, P., Bamberg, E., & Fendler, K. (2003). Charge displacements during ATP-hydrolysis and synthesis of the Na+ -transporting FoF1-ATPase of Ilyobacter tartaricus. Biophysical Journal, 85(3), 2044-2054. doi:10.1016/S0006-3495(03)74632-3.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0024-DBCE-8
Abstract
Transient electrical currents generated by the Na+-transporting FoF1-ATPase of Ilyobacter tartaricus were observed in the hydrolytic and synthetic mode of the enzyme. Two techniques were applied: a photochemical ATP concentration jump on a planar lipid membrane and a rapid solution exchange on a solid supported membrane. We have identified an electrogenic reaction in the reaction cycle of the FoF1-ATPase that is related to the translocation of the cation through the membrane bound Fo subcomplex of the ATPase. In addition, we have determined rate constants for the process: For ATP hydrolysis this reaction has a rate constant of 15–30 s−1 if H+ is transported and 30–60 s−1 if Na+ is transported. For ATP synthesis the rate constant is 50–70 s−1.