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Journal Article

Channelrhodopsin-1: A light-gated proton channel in green algae

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Nagel,  Georg
Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;

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Ollig,  Doris
Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;

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Bamberg,  Ernst
Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Nagel, G., Ollig, D., Fuhrmann, M., Kateriya, S., Mustl, A. M., Bamberg, E., et al. (2002). Channelrhodopsin-1: A light-gated proton channel in green algae. Science, 296(5577), 2395-2398. doi:10.1126/science.1072068.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0024-DC52-3
Abstract
Phototaxis and photophobic responses of green algae are mediated by rhodopsins with microbial-type chromophores. We report a complementary DNA sequence in the green alga Chlamydomonas reinhardtii that encodes a microbial opsin- related protein, which we term Channelopsin-1. The hydrophobic core region of the protein shows homology to the light- activated proton pump bacteriorhodopsin. Expression of Channelopsin-1, or only the hydrophobic core, in Xenopus laevis oocytes in the presence of all-trans retinal produces a light- gated conductance that shows characteristics of a channel selectively permeable for protons. We suggest that Channelrhodopsins are involved in phototaxis of green algae.