English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
Add to Basket
  Local TagsRelease HistoryDetailsSummary

Released
Journal Article

Symmetry embedding in the reconstruction of macromolecular assemblies via the discrete Radon transform

MPS-Authors
/persons/resource/persons137844

Radermacher,  Michael
Department of Structural Biology, Max Planck Institute of Biophysics, Max Planck Society;

External Resource
No external resources are shared
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Lanzavecchia, S., Cantele, F., Radermacher, M., & Bellon, P. L. (2002). Symmetry embedding in the reconstruction of macromolecular assemblies via the discrete Radon transform. Journal of Structural Biology, 137(3), 259-272. doi:10.1016/s1047-8477(02)00004-7.


Cite as: https://hdl.handle.net/11858/00-001M-0000-0024-DC9D-E
Abstract
In this paper we discuss the embedding of symmetry information in an algorithm for three-dimensional reconstruction, which is based on the discrete Radon transform. The original algorithm was designed for randomly oriented and in principal asymmetric particles. The expanded version presented here covers all symmetry point groups which can be exhibited by macromolecular protein assemblies. The orientations of all symmetry equivalent projections, based on the orientation of an experimental projection, are obtained using global group operators. Further, an improved interpolation scheme for the recovery of the three-dimensional discrete Radon transform has been designed for greater computational efficiency. The algorithm has been tested on phantom structures as well as on real data, a virus structure possessing icosahedral symmetry.