Visualizing a protein quake with time-resolved X-ray scattering at a 
free-electron laser

Arnlund, David; Johansson, Linda C; Wickstrand, Cecilia; Barty, Anton; Williams, Garth J; Malmerberg, Erik; Davidsson, Jan; Milathianaki, Despina; DePonte, Daniel P; Shoeman, Robert L.; Wang, Dingjie; James, Daniel; Katona, Gergely; Westenhoff, Sebastian; White, Thomas A; Aquila, Andrew; Bari, Sadia; Berntsen, Peter; Bogan, Mike; van Driel, Tim Brandt; Doak, R. Bruce; Kjær, Kasper Skov; Frank, Matthias; Fromme, Raimund; Grotjohann, Ingo; Henning, Robert; Hunter, Mark S; Kirian, Richard A; Kosheleva, Irina; Kupitz, Christopher; Liang, Mengning; Martin, Andrew V; Nielsen, Martin Meedom; Messerschmidt, Marc; Seibert, M Marvin; Sjöhamn, Jennie; Stellato, Francesco; Weierstall, Uwe; Zatsepin, Nadia A; Spence, John C H; Fromme, Petra; Schlichting, Ilme; Boutet, Sébastien; Groenhof, Gerrit; Chapman, Henry N; Neutze, Richard

doi:10.1038/nmeth.3067

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Visualizing a protein quake with time-resolved X-ray scattering at a free-electron laser

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Shoeman, Robert L.
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

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Doak, R. Bruce
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

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Schlichting, Ilme
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

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http://www.nature.com/nmeth/journal/v11/n9/pdf/nmeth.3067.pdf
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http://dx.doi.org/10.1038/nmeth.3067
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Citation

Arnlund, D., Johansson, L. C., Wickstrand, C., Barty, A., Williams, G. J., Malmerberg, E., et al. (2014). Visualizing a protein quake with time-resolved X-ray scattering at a free-electron laser. Nature methods, 11(9), 923-926. doi:10.1038/nmeth.3067.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0024-9673-0

Abstract

We describe a method to measure ultrafast protein structural changes using time-resolved wide-angle X-ray scattering at an X-ray free-electron laser. We demonstrated this approach using multiphoton excitation of the Blastochloris viridis photosynthetic reaction center, observing an ultrafast global conformational change that arises within picoseconds and precedes the propagation of heat through the protein. This provides direct structural evidence for a 'protein quake': the hypothesis that proteins rapidly dissipate energy through quake-like structural motions.