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Ultrafast extraction of proteins from tissues using desorption by impulsive vibrational excitation

MPS-Authors
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Ren,  Ling
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;

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Kruber,  S.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;

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Robertson,  W.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;

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Miller,  R. J. D.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;

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anie_201407669_sm_miscellaneous_information.pdf
(Supplementary material), 705KB

Citation

Kwiatkowski, M., Wurlitzer, M., Omidi, M., Ren, L., Kruber, S., Nimer, R., et al. (2015). Ultrafast extraction of proteins from tissues using desorption by impulsive vibrational excitation. Angewandte Chemie International Edition, 54(1), 285-288. doi:10.1002/anie.201407669.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0024-C174-E
Abstract
A picosecond IR laser (PIRL) can be used to blast proteins out of tissues through desorption by impulsive excitation (DIVE) of intramolecular vibrational states of water molecules in the cell in less than a millisecond. With PIRL-DIVE proteins covering a range of a few kDa up to several MDa are extracted in high quantities compared to conventional approaches. The chemical composition of extracted proteins remains unaltered and even enzymatic activities are maintained.