English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Alouatta trichromatic color vision: Cone spectra and physiological responses studied with microspectrophotometry and single unit retinal electrophysiology.

MPS-Authors
/persons/resource/persons36469

Lee,  B. B.
Emeritus Group of Membrane Biophysics, MPI for Biophysical Chemistry, Max Planck Society;

External Resource
No external resources are shared
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)

2087474.pdf
(Publisher version), 1005KB

Supplementary Material (public)
There is no public supplementary material available
Citation

Silveira, L. C. L., Saito, C. A., da Silva Filho, M., Kremers, J., Bowmaker, J. K., & Lee, B. B. (2014). Alouatta trichromatic color vision: Cone spectra and physiological responses studied with microspectrophotometry and single unit retinal electrophysiology. PLoS One, 9(11): e113321. doi:10.1371/journal.pone.0113321.


Cite as: https://hdl.handle.net/11858/00-001M-0000-0024-B638-2
Abstract
The howler monkeys (Alouatta sp.) are the only New World primates to exhibit routine trichromacy. Both males and females have three cone photopigments. However, in contrast to Old World monkeys, Alouatta has a locus control region upstream of each opsin gene on the X-chromosome and this might influence the retinal organization underlying its color vision. Postmortem microspectrophotometry (MSP) was performed on the retinae of two male Alouatta to obtain rod and cone spectral sensitivities. The MSP data were consistent with only a single opsin being expressed in each cone and electrophysiological data were consistent with this primate expressing full trichromacy. To study the physiological organization of the retina underlying Alouatta trichromacy, we recorded from retinal ganglion cells of the same animals used for MSP measurements with a variety of achromatic and chromatic stimulus protocols. We found MC cells and PC cells in the Alouatta retina with similar properties to those previously found in the retina of other trichromatic primates. MC cells showed strong phasic responses to luminance changes and little response to chromatic pulses. PC cells showed strong tonic response to chromatic changes and small tonic response to luminance changes. Responses to other stimulus protocols (flicker photometry; changing the relative phase of red and green modulated lights; temporal modulation transfer functions) were also similar to those recorded in other trichromatic primates. MC cells also showed a pronounced frequency double response to chromatic modulation, and with luminance modulation response saturation accompanied by a phase advance between 10-20 Hz, characteristic of a contrast gain mechanism. This indicates a very similar retinal organization to Old-World monkeys. Cone-specific opsin expression in the presence of a locus control region for each opsin may call into question the hypothesis that this region exclusively controls opsin expression.