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Dual channel RESOLFT nanoscopy by using fluorescent state kinetics.

MPS-Authors
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Testa,  I.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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D´Este,  E.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Urban,  N. T.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Balzarotti,  F.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Hell,  S. W.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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2096446_Suppl.pdf
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Citation

Testa, I., D´Este, E., Urban, N. T., Balzarotti, F., & Hell, S. W. (2015). Dual channel RESOLFT nanoscopy by using fluorescent state kinetics. Nano Letters, 15(1), 103-106. doi:10.1021/nl503058k.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0024-CEA3-D
Abstract
We show that RESOLFT fluorescence nanoscopy, a low light level scanning superresolution technique employing reversibly switchable fluorescent proteins (rsFPs), is capable of dual-channel live-cell imaging that is virtually free of chromatic errors and temporal offsets. This is accomplished using rsEGFP and Dronpa, two rsFPs having similar spectra but different kinetics of switching and fluorescence emission. Our approach is demonstrated by imaging protein distributions and dynamics in living neurons and neuronal tissues.