Dual channel RESOLFT nanoscopy by using fluorescent state kinetics.

Testa, I.; D´Este, E.; Urban, N. T.; Balzarotti, F.; Hell, S. W.

doi:10.1021/nl503058k

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学術論文

Dual channel RESOLFT nanoscopy by using fluorescent state kinetics.

MPS-Authors

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Testa, I.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons141821

D´Este, E.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons39413

Urban, N. T.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons141819

Balzarotti, F.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons15210

Hell, S. W.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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http://pubs.acs.org/doi/ipdf/10.1021/nl503058k
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引用

Testa, I., D´Este, E., Urban, N. T., Balzarotti, F., & Hell, S. W. (2015). Dual channel RESOLFT nanoscopy by using fluorescent state kinetics. Nano Letters, 15(1), 103-106. doi:10.1021/nl503058k.

引用: https://hdl.handle.net/11858/00-001M-0000-0024-CEA3-D

要旨

We show that RESOLFT fluorescence nanoscopy, a low light level scanning superresolution technique employing reversibly switchable fluorescent proteins (rsFPs), is capable of dual-channel live-cell imaging that is virtually free of chromatic errors and temporal offsets. This is accomplished using rsEGFP and Dronpa, two rsFPs having similar spectra but different kinetics of switching and fluorescence emission. Our approach is demonstrated by imaging protein distributions and dynamics in living neurons and neuronal tissues.