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Journal Article

STED nanoscopy reveals the ubiquity of subcortical cytoskeleton periodicity in living neurons.

MPS-Authors
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D'Este,  E.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

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Kamin,  D.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

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Göttfert,  F.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

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El-Hady Hashem,  A.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

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Hell,  S. W.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

Fulltext (public)

2105160.pdf
(Publisher version), 3MB

Supplementary Material (public)

2105160_Suppl_1.pdf
(Supplementary material), 700KB

2105160_Suppl_2.pdf
(Supplementary material), 4MB

Citation

D'Este, E., Kamin, D., Göttfert, F., El-Hady Hashem, A., & Hell, S. W. (2015). STED nanoscopy reveals the ubiquity of subcortical cytoskeleton periodicity in living neurons. Cell Reports, 10(8), 1246-1251. doi:10.1016/j.celrep.2015.02.007.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0025-05F3-D
Abstract
In the axons of cultured hippocampal neurons, actin forms various structures, including bundles, patches (involved in the preservation of neuronal polarity), and a recently reported periodic ring-like structure. Nevertheless, the overlaying organization of actin in neurons and in the axon initial segment (AIS) is still unclear, due mainly to a lack of adequate imaging methods. By harnessing live-cell stimulated emission depletion (STED) nanoscopy and the fluorescent probe SiR-Actin, we show that the periodic subcortical actin structure is in fact present in both axons and dendrites. The periodic cytoskeleton organization is also found in the peripheral nervous system, specifically at the nodes of Ranvier. The actin patches in the AIS co-localize with pre-synaptic markers. Cytosolic actin organization strongly depends on the developmental stage and subcellular localization. Altogether, the results of this study reveal unique neuronal cytoskeletal features.