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Ctk1 Function Is Necessary for Full Translation Initiation Activity in Saccharomyces cerevisiae

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Soufi,  Boumediene
Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society;

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Olsen,  Jesper V.
Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society;

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Citation

Coordes, B., Brünger, K. M., Burger, K., Soufi, B., Horenk, J., Eick, D., et al. (2015). Ctk1 Function Is Necessary for Full Translation Initiation Activity in Saccharomyces cerevisiae. EUKARYOTIC CELL, 14(1), 86-95. doi:10.1128/EC.00106-14.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0025-6D52-8
Abstract
Translation is a fundamental and highly regulated cellular process. Previously, we reported that the kinase and transcription elongation factor Ctk1 increases fidelity during translation elongation in Saccharomyces cerevisiae. Here, we show that loss of Ctk1 function also affects the initiation step of translation. Translation active extracts from Ctk1-depleted cells show impaired translation activity of capped mRNA, but not mRNA reporters containing the cricket paralysis virus (CrPV) internal ribosome entry site (IRES). Furthermore, the formation of 80S initiation complexes is decreased, which is probably due to reduced subunit joining. In addition, we determined the changes in the phosphorylation pattern of a ribosome enriched fraction after depletion of Ctk1. Thus, we provide a catalogue of phosphoproteomic changes dependent on Ctk1. Taken together, our data suggest a stimulatory function of Ctk1 in 80S formation during translation initiation.