Architecture of the RNA polymerase II–Mediator core initiation complex.

Plaschka, C.; Larivière, L.; Wenzeck, L.; Seizl, M.; Hemann, M.; Tegunov, D.; Petrotchenko, E. V.; Borchers, C. H.; Baumeister, W.; Herzog, F.; Villa, E.; Cramer, P.

doi:10.1038/nature14229

アイテム詳細

登録内容を編集ファイル形式で保存

一時保存へ追加

タグ情報を表示リリース履歴を表示詳細要約

公開

学術論文

Architecture of the RNA polymerase II–Mediator core initiation complex.

MPS-Authors

/persons/resource/persons145292

Tegunov, D.
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons77721

Baumeister, W.
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons78832

Villa, E.
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

External Resource

There are no locators available

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

フルテキスト (公開)

公開されているフルテキストはありません

付随資料 (公開)

There is no public supplementary material available

引用

Plaschka, C., Larivière, L., Wenzeck, L., Seizl, M., Hemann, M., Tegunov, D., Petrotchenko, E. V., Borchers, C. H., Baumeister, W., Herzog, F., Villa, E., & Cramer, P. (2015). Architecture of the RNA polymerase II–Mediator core initiation complex. Nature, 518, 376-380. doi:10.1038/nature14229.

引用: https://hdl.handle.net/11858/00-001M-0000-0025-7671-C

要旨

The conserved co-activator complex Mediator enables regulated transcription initiation by RNA polymerase (Pol) II. Here we reconstitute an active 15-subunit core Mediator (cMed) comprising all essential Mediator subunits from Saccharomyces cerevisiae. The cryo-electron microscopic structure of cMed bound to a core initiation complex was determined at 9.7 Å resolution. cMed binds Pol II around the Rpb4–Rpb7 stalk near the carboxy-terminal domain (CTD). The Mediator head module binds the Pol II dock and the TFIIB ribbon and stabilizes the initiation complex. The Mediator middle module extends to the Pol II foot with a ‘plank’ that may influence polymerase conformation. The Mediator subunit Med14 forms a ‘beam’ between the head and middle modules and connects to the tail module that is predicted to bind transcription activators located on upstream DNA. The Mediator ‘arm’ and ‘hook’ domains contribute to a ‘cradle’ that may position the CTD and TFIIH kinase to stimulate Pol II phosphorylation.