日本語
 
User Manual Privacy Policy ポリシー/免責事項 連絡先
  詳細検索ブラウズ

アイテム詳細


公開

学術論文

Fluorescence Correlation Spectroscopy: Principles and Applications

MPS-Authors
/persons/resource/persons15815

Schwille,  Petra
Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society;

URL
There are no locators available
フルテキスト (公開)
公開されているフルテキストはありません
付随資料 (公開)
There is no public supplementary material available
引用

Bacia, K., Haustein, E., & Schwille, P. (2014). Fluorescence Correlation Spectroscopy: Principles and Applications. Cold Spring Harbor Protocols, 709-725. doi:10.1101/pdb.top081802.


引用: http://hdl.handle.net/11858/00-001M-0000-0025-79B6-F
要旨
Fluorescence correlation spectroscopy (FCS) is used to study the movements and the interactions of biomolecules at extremely dilute concentrations, yielding results with good spatial and temporal resolutions. Using a number of technical developments, FCS has become a versatile technique that can be used to study a variety of sample types and can be advantageously combined with other methods. Unlike other fl uorescence-based techniques, the analysis of FCS data is not based on the average intensity of the fl uorescence emission but examines the minute intensity fl uctuations caused by sponta- neous deviations from the mean at thermal equilibrium. These fl uctuations can result from variations in local concentrations owing to molecular mobility or from characteristic intermolecular or intramo- lecular reactions of fl uorescently labeled biomolecules present at low concentrations. Here, we provide a basic introduction to FCS, including its technical development and theoretical basis, experimental setup of an FCS system, adjustment of a setup, data acquisition, and analysis of FCS measurements. Finally, the application of FCS to the study of lipid bilayer membranes and to living cells is discussed.