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Journal Article

Systemic cytosolic Ca2+ elevation is activated upon wounding and herbivory in Arabidopsis

MPS-Authors
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Vadassery,  Jyothilakshmi
Department of Bioorganic Chemistry, Prof. Dr. W. Boland, MPI for Chemical Ecology, Max Planck Society;

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Boland,  Wilhelm
Department of Bioorganic Chemistry, Prof. Dr. W. Boland, MPI for Chemical Ecology, Max Planck Society;

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Mithöfer,  Axel
Department of Bioorganic Chemistry, Prof. Dr. W. Boland, MPI for Chemical Ecology, Max Planck Society;
Research Group Dr. A. Mithöfer, Plant Defense Physiology, Department of Bioorganic Chemistry, Prof. Dr. W. Boland, MPI for Chemical Ecology, Max Planck Society;

External Resource

https://doi.org/10.1111/nph.13493
(Publisher version)

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Citation

Kiep, V., Vadassery, J., Lattke, J., Maaß, J.-P., Boland, W., Peiter, E., et al. (2015). Systemic cytosolic Ca2+ elevation is activated upon wounding and herbivory in Arabidopsis. New Phytologist, 207(4), 996-1004. doi:10.1111/nph.13493.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0026-C935-C
Abstract
Calcium ion (Ca2+) signalling triggered by insect herbivory is an intricate network with multiple components, involving positive and negative regulators. Real-time, noninvasive imaging of entire Arabidopsis thaliana rosettes was employed to monitor cytosolic free calcium ([Ca2+]cyt) elevations in local and systemic leaves in response to wounding and Spodoptera littoralis feeding. Luminescence emitted by the cytosol-localized Ca2+ reporter aequorin was imaged using a high-resolution photon-counting camera system. Spodoptera littoralis feeding on Arabidopsis induced both local and systemic [Ca2+]cyt elevations. Systemic [Ca2+]cyt signals were found predominantly in adjacent leaves with direct vascular connections to the treated leaf and appeared with a delay of 1 to 2 min. Simulated herbivory by wounding always induced a local [Ca2+]cyt response, but a systemic one only when the midrib was wounded. This systemic [Ca2+]cyt response was suppressed by the presence of insect-derived oral secretions as well as in a mutant of the vacuolar cation channel, Two Pore Channel 1 (TPC1). Our results provide evidence that in Arabidopsis insect herbivory induces both local and systemic [Ca2+]cyt signals that distribute within the vascular system. The systemic [Ca2+]cyt signal could play an important signalling role in systemic plant defence.