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Long-tip high-speed atomic force microscopy for nanometer-scale imaging in live cells

MPS-Authors

Shibata,  Mikihiro
Max Planck Florida Institute for Neuroscience, Max Planck Society;

Uchihashi,  Takayuki
Max Planck Florida Institute for Neuroscience, Max Planck Society;

Ando,  Toshio
Max Planck Florida Institute for Neuroscience, Max Planck Society;

Yasuda,  Ryohei
Max Planck Florida Institute for Neuroscience, Max Planck Society;

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Citation

Shibata, M., Uchihashi, T., Ando, T., & Yasuda, R. (2015). Long-tip high-speed atomic force microscopy for nanometer-scale imaging in live cells. Scientific Reports, 5, 8724.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0026-D075-E
Abstract
Visualization of morphological dynamics of live cells with nanometer resolution under physiological conditions is highly desired, but challenging. It has been demonstrated that high-speed atomic force microscopy is a powerful technique for visualizing dynamics of biomolecules under physiological conditions. However, application of high-speed atomic force microscopy for imaging larger objects such as live mammalian cells has been complicated because of the collision between the cantilever and samples. Here, we demonstrate that attaching an extremely long (~3 μm) and thin (~5 nm) tip by amorphous carbon to the cantilever allows us to image the surface structure of live cells with the spatiotemporal resolution of nanometers and seconds. We demonstrate that long-tip high-speed atomic force microscopy is capable of imaging morphogenesis of filopodia, membrane ruffles, pit formation, and endocytosis in COS-7, HeLa cells and hippocampal neurons.