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Journal Article

STED nanoscopy with fluorescent quantum dots.

MPS-Authors
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Hoyer,  P.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Engelhardt,  J.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Sahl,  S. J.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Hell,  S. W.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

Fulltext (public)

2156719.pdf
(Publisher version), 2MB

Supplementary Material (public)

2156719_Suppl.pdf
(Supplementary material), 3MB

Citation

Hanne, J., Falk, H. J., Görlitz, F., Hoyer, P., Engelhardt, J., Sahl, S. J., et al. (2015). STED nanoscopy with fluorescent quantum dots. Nature Communications, 6: 7127. doi:10.1038/ncomms8127.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0027-1290-2
Abstract
The widely popular class of quantum-dot molecular labels could so far not be utilized as standard fluorescent probes in STED (stimulated emission depletion) nanoscopy. This is because broad quantum-dot excitation spectra extend deeply into the spectral bands used for STED, thus compromising the transient fluorescence silencing required for attaining super-resolution. Here we report the discovery that STED nanoscopy of several red-emitting commercially available quantum dots is in fact successfully realized by the increasingly popular 775 nm STED laser light. A resolution of presently ~50 nm is demonstrated for single quantum dots, and sub-diffraction resolution is further shown for imaging of quantum-dot-labelled vimentin filaments in fibroblasts. The high quantum-dot photostability enables repeated STED recordings with >1,000 frames. In addition, we have evidence that the tendency of quantum-dot labels to blink is largely suppressed by combined action of excitation and STED beams. Quantum-dot STED significantly expands the realm of application of STED nanoscopy, and, given the high stability of these probes, holds promise for extended time-lapse imaging.