Magnetic resonance and optical imaging probes for NMDA receptors on the cell 
surface of neurons: synthesis and evaluation in cellulo

Sim, N; Pal, R; Parker, D; Engelmann, J; Mishra, A; Gottschalk, S

doi:10.1039/C4OB01848F

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Magnetic resonance and optical imaging probes for NMDA receptors on the cell surface of neurons: synthesis and evaluation in cellulo

MPS-Authors

/persons/resource/persons83903

Engelmann, J
Department High-Field Magnetic Resonance, Max Planck Institute for Biological Cybernetics, Max Planck Society;
Max Planck Institute for Biological Cybernetics, Max Planck Society;

/persons/resource/persons83941

Gottschalk, S
Department High-Field Magnetic Resonance, Max Planck Institute for Biological Cybernetics, Max Planck Society;
Max Planck Institute for Biological Cybernetics, Max Planck Society;

External Resource

http://pubs.rsc.org/en/content/getauthorversionpdf/C4OB01848F
(Publisher version)

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Sim, N., Pal, R., Parker, D., Engelmann, J., Mishra, A., & Gottschalk, S. (2014). Magnetic resonance and optical imaging probes for NMDA receptors on the cell surface of neurons: synthesis and evaluation in cellulo. Organic Biomolecular Chemistry, 12(46), 9389-9404. doi:10.1039/C4OB01848F.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0027-7F8B-C

Abstract

A second generation of N-methyl-D-aspartate (NMDA) receptor-targeted MRI contrast agents has been synthesised and evaluated in cellulo, based on established bicyclic NMDA receptor antagonists. Their use as responsive MR imaging probes has been evaluated in suspensions of NSC-34 cells, and one agent exhibited significant enhancements in measured longitudinal and transverse water proton relaxation rates (19 and 38% respectively; 3 T, 298 K). A biotin derivative of the lead compound was prepared and the specificity and reversibility of binding to the NMDA cell surface receptors demonstrated using confocal laser scanning microscopy. Competitive and reversible binding of glutamate to the receptors was also visualised, suggesting that the receptor-targeted approach may allow MRI to be used to monitor neuronal events associated with modulation of local glutamate concentrations.