English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Genome-Wide Association Analyses Point to Candidate Genes for Electric Shock Avoidance in Drosophila melanogaster

MPS-Authors
/persons/resource/persons84672

Appel,  Mirjam
Max Planck Research Group: Behavioral Genetics / Tanimoto, MPI of Neurobiology, Max Planck Society;

/persons/resource/persons80981

Oguz,  Tuba
Max Planck Research Group: Behavioral Genetics / Tanimoto, MPI of Neurobiology, Max Planck Society;

/persons/resource/persons39094

Tanimoto,  Hiromu
Max Planck Research Group: Behavioral Genetics / Tanimoto, MPI of Neurobiology, Max Planck Society;

/persons/resource/persons39126

Yarali,  Ayse
Max Planck Research Group: Behavioral Genetics / Tanimoto, MPI of Neurobiology, Max Planck Society;

External Resource
No external resources are shared
Fulltext (public)

journal.pone.0126986.pdf
(Any fulltext), 939KB

Supplementary Material (public)
There is no public supplementary material available
Citation

Appel, M., Scholz, C.-J., Müller, T., Dittrich, M., König, C., Bockstaller, M., et al. (2015). Genome-Wide Association Analyses Point to Candidate Genes for Electric Shock Avoidance in Drosophila melanogaster. PLOS ONE, 10(5): e0126986. doi:10.1371/journal.pone.0126986.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0027-A63D-5
Abstract
Electric shock is a common stimulus for nociception-research and the most widely used reinforcement in aversive associative learning experiments. Yet, nothing is known about the mechanisms it recruits at the periphery. To help fill this gap, we undertook a genome-wide association analysis using 38 inbred Drosophila melanogaster strains, which avoided shock to varying extents. We identified 514 genes whose expression levels and/or sequences covaried with shock avoidance scores. We independently scrutinized 14 of these genes using mutants, validating the effect of 7 of them on shock avoidance. This emphasizes the value of our candidate gene list as a guide for follow-up research. In addition, by integrating our association results with external protein-protein interaction data we obtained a shock avoidance- associated network of 38 genes. Both this network and the original candidate list contained a substantial number of genes that affect mechanosensory bristles, which are hairlike organs distributed across the fly's body. These results may point to a potential role for mechanosensory bristles in shock sensation. Thus, we not only provide a first list of candidate genes for shock avoidance, but also point to an interesting new hypothesis on nociceptive mechanisms.