Phosphatidylinositol phosphate kinase type Iγ regulates dynamics of large 
dense-core vesicle fusion.

Gong, L. W.; Di Paolo, G.; Diaz, E.; Cestra, G.; Diaz, M. E.; Lindau, M.; De Camilli, P.; Toomre, D.

doi:10.1073/pnas.0501412102

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Journal Article

Phosphatidylinositol phosphate kinase type Iγ regulates dynamics of large dense-core vesicle fusion.

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Lindau, M.
Research Group of Nanoscale Cell Biology, MPI for Biophysical Chemistry, Max Planck Society;

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http://www.pnas.org/content/102/14/5204.full.pdf
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Citation

Gong, L. W., Di Paolo, G., Diaz, E., Cestra, G., Diaz, M. E., Lindau, M., et al. (2005). Phosphatidylinositol phosphate kinase type Iγ regulates dynamics of large dense-core vesicle fusion. Proceedings of the National Academy of Sciences of the United States of America, 102(14), 5204-5209. doi: 10.1073/pnas.0501412102.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0027-AA7D-8

Abstract

Phosphatidylinositol-4,5-bisphosphate was proposed to be an important regulator of large dense-core vesicle exocytosis from neuroendocrine tissues. Here, we have examined the kinetics of secretion in chromaffin cells from mice lacking phosphatidylinositol phosphate kinase type Iγ, the major neuronal phosphatidylinositol-4-phosphate 5-kinase. Absence of this enzyme caused a reduction of the readily releasable vesicle pool and its refilling rate, with a small increase in morphologically docked vesicles, indicating a defect in vesicle priming. Furthermore, amperometry revealed a delay in fusion pore expansion. These results provide direct genetic evidence for a key role of phosphatidylinositol-4,5-bisphosphate synthesis in the regulation of large dense-core vesicle fusion dynamics.