English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Experimental Evolution of Species Recognition

MPS-Authors
/persons/resource/persons56565

Rogers,  David W.
Max-Planck Research Group Experimental Evolution, Max Planck Institute for Evolutionary Biology, Max Planck Society;

/persons/resource/persons56646

Denton,  Jai A.
Max-Planck Research Group Experimental Evolution, Max Planck Institute for Evolutionary Biology, Max Planck Society;

/persons/resource/persons56564

McConnell,  Ellen
Max-Planck Research Group Experimental Evolution, Max Planck Institute for Evolutionary Biology, Max Planck Society;

/persons/resource/persons56563

Greig,  Duncan
Max-Planck Research Group Experimental Evolution, Max Planck Institute for Evolutionary Biology, Max Planck Society;

External Resource
No external resources are shared
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Rogers, D. W., Denton, J. A., McConnell, E., & Greig, D. (2015). Experimental Evolution of Species Recognition. Current Biology, 25(13), 1753-1758. doi:10.1016/j.cub.2015.05.023.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0027-AAB7-6
Abstract
Sex with another species can be disastrous, especially for organisms that mate only once, like yeast [1, 2 and 3]. Courtship signals, including pheromones, often differ between species and can provide a basis for distinguishing between reproductively compatible and incompatible partners [4, 5 and 6]. Remarkably, we show that the baker’s yeast Saccharomyces cerevisiae does not reject mates engineered to produce pheromones from highly diverged species, including species that have been reproductively isolated for up to 100 million years. To determine whether effective discrimination against mates producing pheromones from other species is possible, we experimentally evolved pheromone receptors under conditions that imposed high fitness costs on mating with cells producing diverged pheromones. Evolved receptors allowed both efficient mating with cells producing the S. cerevisiae pheromone and near-perfect discrimination against cells producing diverged pheromones. Sequencing evolved receptors revealed that each contained multiple mutations that altered the amino acid sequence. By isolating individual mutations, we identified specific amino acid changes that dramatically improved discrimination. However, the improved discrimination conferred by these individual mutations came at the cost of reduced mating efficiency with cells producing the S. cerevisiae pheromone, resulting in low fitness. This tradeoff could be overcome by simultaneous introduction of separate mutations that improved mating efficiency alongside those that improved discrimination. Thus, if mutations occur sequentially, the shape of the fitness landscape may prevent evolution of the optimal phenotype [ 7 and 8]—offering a possible explanation for the poor discrimination of receptors found in nature.