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Journal Article

Cell staining by photo-activated dye and its conjugate with Chitosan.


Möbius,  D.
Research Group of Molecular Organized Systems, MPI for biophysical chemistry, Max Planck Society;

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Zaitsev, S. Y., Shaposhnikov, M. N., Solovyeva, D. O., Zaitsev, I. S., & Möbius, D. (2015). Cell staining by photo-activated dye and its conjugate with Chitosan. Cell Biochemistry and Biophysics, 71(3), 1475-1481. doi:10.1007/s12013-014-0370-1.

Cite as: http://hdl.handle.net/11858/00-001M-0000-0027-AFBD-0
Photo-activated or “Caged” rhodamine dyes are the most useful for microscopic investigation of biological tissue by various fluorescent techniques. Novel precursor of the fluorescent dye (PFD813) has been studied for photosensitive staining of numerous animal cells. The functional rhodamine dye (Rho813) with intensive fluorescence has been obtained after photoactivation of its precursor PFD813 inside cells. The dye Rho813 has been successfully used for the optical detection of particular features in biological objects (HaCaT cells, HBL-100, MDCK, lymphocytes). Moreover, the chitosan conjugate with PFD molecules (“Chitosan-PFD813″) has been obtained and studied for the first time. The developed procedures and obtained data are important for further applications of novel precursors of fluorescent dyes (“caged” dyes) for microscopic probing of biological objects. As example, the synthesized “Chitosan-PFD813″ has been successfully applied in this study for intracellular transport visualization by fluorescent microscopy. Cell Biochemistry and Biophysics Cell Biochemistry and Biophysics Look Inside Other actions Export citation Register for Journal Updates About This Journal Reprints and Permissions Add to Papers Share Share this content on Facebook Share this content on Twitter Share this content on LinkedIn