English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

High speed optical coherence microscopy with autofocus adjustment and a miniaturized endoscopic imaging probe

MPS-Authors
/persons/resource/persons95132

Sawinski,  Jürgen
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons128986

Denk,  Winfried
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

Fulltext (public)
There are no public fulltexts available
Supplementary Material (public)
There is no public supplementary material available
Citation

Aguirre, A. D., Sawinski, J., Huang, S. W., Zhou, C., Denk, W., & Fujimoto, J. G. (2010). High speed optical coherence microscopy with autofocus adjustment and a miniaturized endoscopic imaging probe. Optics Express, 18(5), 4222-4239. doi:10.1364/OE.18.004222.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0024-2092-C
Abstract
Optical coherence microscopy (OCM) is a promising technique for high resolution cellular imaging in human tissues. An OCM system for high−speed en face cellular resolution imaging was developed at 1060 nm wavelength at frame rates up to 5 Hz with resolutions of < 4 microm axial and < 2 microm transverse. The system utilized a novel polarization compensation method to combat wavelength dependent source polarization and achieve broadband electro−optic phase modulation compatible with ultrahigh axial resolution. In addition, the system incorporated an auto−focusing feature that enables precise, near real−time alignment of the confocal and coherence gates in tissue, allowing user−friendly optimization of image quality during the imaging procedure. Ex vivo cellular images of human esophagus, colon, and cervix as well as in vivo results from human skin are presented. Finally, the system design is demonstrated with a miniaturized piezoelectric fiber−scanning probe which can be adapted for laparoscopic and endoscopic imaging applications