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Journal Article

Two-photon imaging to a depth of 1000 µm microm in living brains by use of a Ti:Al2O3 regenerative amplifier

MPS-Authors
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Theer,  Patrick
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

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Hasan,  Mazahir T.
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

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Denk,  Winfried
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

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Citation

Theer, P., Hasan, M. T., & Denk, W. (2003). Two-photon imaging to a depth of 1000 µm microm in living brains by use of a Ti:Al2O3 regenerative amplifier. Optics Letters, 28(12), 1022-1024. doi:10.1364/OL.28.001022.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0019-9E28-5
Abstract
It is shown that two−photon fluorescence images can be obtained throughout almost the entire gray matter of the mouse neocortex by using optically amplified femtosecond pulses. The achieved imaging depth approaches the theoretical limit set by excitation of out−of−focus fluorescence