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Journal Article

Ultrafast temporally stochastic STED nanoscopy of millisecond dynamics.

MPS-Authors
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Sahl,  S.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Engelhardt,  J.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons15210

Hell,  S. W.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

External Resource

http://www.nature.com/nmeth/journal/v12/n9/pdf/nmeth.3481.pdf
(Publisher version)

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Supplementary Material (public)

2173123_Suppl_1.html
(Supplementary material), 74KB

2173123_Suppl_2.html
(Supplementary material), 72KB

2173123_Suppl_3.html
(Supplementary material), 73KB

2173123_Suppl_4.html
(Supplementary material), 78KB

2173123-Suppl_5.html
(Supplementary material), 78KB

2173123_Suppl_6.html
(Supplementary material), 78KB

2173123_Suppl_7.html
(Supplementary material), 78KB

2173123_Suppl_8.html
(Supplementary material), 78KB

2173123_Suppl_9.pdf
(Supplementary material), 787KB

Citation

Schneider, J., Zahn, J., Maglione, M., Sigrist, S. J., Marquard, J., Chojnacki, J., et al. (2015). Ultrafast temporally stochastic STED nanoscopy of millisecond dynamics. Nature Methods, 12(9), 827-830. doi:10.1038/nmeth.3481.


Cite as: https://hdl.handle.net/11858/00-001M-0000-0027-D460-F
Abstract
Electro-optical scanning (>1,000 frames/s) with pixel dwell times on the order of the lifetime of the fluorescent molecular state renders stimulated emission depletion (STED) nanoscopy temporally stochastic. Photon detection from a molecule occurs stochastically in one of several scanning frames, and the spatial origin of the photon is known with subdiffraction precision. Images are built up by binning consecutive frames, making the time resolution freely adjustable. We demonstrated nanoscopy of vesicle motions in living Drosophila larvae and the cellular uptake of viral particles with 5- to 10-ms temporal resolution.