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Microfluidic tools for quantitative studies of eukaryotic chemotaxis

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Beta,  Carsten
Laboratory for Fluid Dynamics, Pattern Formation and Biocomplexity, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society;

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Bodenschatz,  Eberhard
Laboratory for Fluid Dynamics, Pattern Formation and Biocomplexity, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society;

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Beta, C., & Bodenschatz, E. (2011). Microfluidic tools for quantitative studies of eukaryotic chemotaxis. European Journal of Cell Biology, 90, 811-816. doi:10.1016/j.ejcb.2011.05.006.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0029-115F-F
Abstract
Over the past decade, microfluidic techniques have been established as a versatile platform to perform live cell experiments under well-controlled conditions. To investigate the directional responses of cells, stable concentration profiles of chemotactic factors can be generated in microfluidic gradient mixers that provide a high degree of spatial control. However, the times for built-up and switching of gradient profiles are in general too slow to resolve the intracellular protein translocation events of directional sensing of eukaryotes. Here, we review an example of a conventional microfluidic gradient mixer as well as the novel flow photolysis technique that achieves an increased temporal resolution by combining the photo-activation of caged compounds with the advantages of microfluidic chambers.