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Journal Article

Live cell flattening - traditional and novel approaches

MPS-Authors
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Westendorf,  Christian
Laboratory for Fluid Dynamics, Pattern Formation and Biocomplexity, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society;

/persons/resource/persons173453

Bae,  Albert
Laboratory for Fluid Dynamics, Pattern Formation and Biocomplexity, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society;

/persons/resource/persons173472

Bodenschatz,  Eberhard
Laboratory for Fluid Dynamics, Pattern Formation and Biocomplexity, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society;

/persons/resource/persons21364

Beta,  Carsten
Laboratory for Fluid Dynamics, Pattern Formation and Biocomplexity, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society;

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Citation

Westendorf, C., Bae, A., Erlenkamper, C., Galland, E., Franck, C., Bodenschatz, E., et al. (2010). Live cell flattening - traditional and novel approaches. PMC Biophysics, 3, 9-1-9-15. doi:10.1186/1757-5036-3-9.


Cite as: https://hdl.handle.net/11858/00-001M-0000-0029-126F-3
Abstract
Eukaryotic cell flattening is valuable for improving microscopic observations, ranging from bright field (BF) to total internal reflection fluorescence (TIRF) microscopy. Fundamental processes, such as mitosis and in vivo actin polymerization, have been investigated using these techniques. Here, we review the well known agar overlayer protocol and the oil overlay method. In addition, we present more elaborate microfluidics-based techniques that provide us with a greater level of control. We demonstrate these techniques on the social amoebae Dictyostelium discoideum, comparing the advantages and disadvantages of each method.