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Developmental profile of kainate receptor subunit KA1 revealed by Cre expression in YAC transgenic mice

MPG-Autoren
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Jerecic,  Jasna
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

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Zamanillo,  Daniel
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

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Wilbertz,  Johannes
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;

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Sprengel,  Rolf
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

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Seeburg,  Peter H.
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

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Zitation

Kask, K., Jerecic, J., Zamanillo, D., Wilbertz, J., Sprengel, R., & Seeburg, P. H. (2000). Developmental profile of kainate receptor subunit KA1 revealed by Cre expression in YAC transgenic mice. Brain Research, 876(1-2), 55-61. doi:10.1016/S0006-8993(00)02599-3.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0028-2D32-5
Zusammenfassung
To determine the spatio-temporal expression in brain of the high-affinity kainate receptor subunit KA1, we generated transgenic mice expressing Cre recombinase from the KA1 gene on a chromosomally integrated 550 kb yeast artificial chromosome (YAC). Activity of the KA1 gene promoter during brain development was visualized by Cre immunohistochemistry, and by X-gal staining of b-galactosidase induced by Cre recombinase in double transgenic KA1-Cre/lacZ indicator mice. During early brain development, expression from the YAC-carried KA1-Cre transgene was observed in all major brain areas, predicting a function for KA1 in the developing central nervous system. In the adult brain, KA1-Cre transgene expression was restricted mainly to hippocampal CA3 pyramidal and dentate gyrus granule cells, an adult expression pattern characteristic for the endogenous KA1 alleles. KA1-Cre transgenic mice may help in elucidating the role of floxed genes ablated in vivo in KA1 expressing neurons