English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
Add to Basket
  Local TagsRelease HistoryDetailsSummary

Released
Journal Article

Developmental profile of kainate receptor subunit KA1 revealed by Cre expression in YAC transgenic mice

MPS-Authors
/persons/resource/persons93606

Jerecic,  Jasna
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons96058

Zamanillo,  Daniel
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons95936

Wilbertz,  Johannes
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons95439

Sprengel,  Rolf
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons95292

Seeburg,  Peter H.
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

External Resource

http://www.sciencedirect.com/science/article/pii/S0006899300025993/pdf?md5=ddd56d3dc609b707c33121bcd2e97b4a&pid=1-s2.0-S0006899300025993-main.pdf
(Any fulltext)

http://dx.doi.org/10.1016/S0006-8993(00)02599-3
(Any fulltext)

Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Kask, K., Jerecic, J., Zamanillo, D., Wilbertz, J., Sprengel, R., & Seeburg, P. H. (2000). Developmental profile of kainate receptor subunit KA1 revealed by Cre expression in YAC transgenic mice. Brain Research, 876(1-2), 55-61. doi:10.1016/S0006-8993(00)02599-3.


Cite as: https://hdl.handle.net/11858/00-001M-0000-0028-2D32-5
Abstract
To determine the spatio-temporal expression in brain of the high-affinity kainate receptor subunit KA1, we generated transgenic mice expressing Cre recombinase from the KA1 gene on a chromosomally integrated 550 kb yeast artificial chromosome (YAC). Activity of the KA1 gene promoter during brain development was visualized by Cre immunohistochemistry, and by X-gal staining of b-galactosidase induced by Cre recombinase in double transgenic KA1-Cre/lacZ indicator mice. During early brain development, expression from the YAC-carried KA1-Cre transgene was observed in all major brain areas, predicting a function for KA1 in the developing central nervous system. In the adult brain, KA1-Cre transgene expression was restricted mainly to hippocampal CA3 pyramidal and dentate gyrus granule cells, an adult expression pattern characteristic for the endogenous KA1 alleles. KA1-Cre transgenic mice may help in elucidating the role of floxed genes ablated in vivo in KA1 expressing neurons