Developmental profile of kainate receptor subunit KA1 revealed by Cre 
expression in YAC transgenic mice

Kask, Kalev; Jerecic, Jasna; Zamanillo, Daniel; Wilbertz, Johannes; Sprengel, Rolf; Seeburg, Peter H.

doi:10.1016/S0006-8993(00)02599-3

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学術論文

Developmental profile of kainate receptor subunit KA1 revealed by Cre expression in YAC transgenic mice

MPS-Authors

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Jerecic, Jasna
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons96058

Zamanillo, Daniel
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

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Wilbertz, Johannes
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons95439

Sprengel, Rolf
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

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Seeburg, Peter H.
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

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http://www.sciencedirect.com/science/article/pii/S0006899300025993/pdf?md5=ddd56d3dc609b707c33121bcd2e97b4a&pid=1-s2.0-S0006899300025993-main.pdf
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http://dx.doi.org/10.1016/S0006-8993(00)02599-3
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引用

Kask, K., Jerecic, J., Zamanillo, D., Wilbertz, J., Sprengel, R., & Seeburg, P. H. (2000). Developmental profile of kainate receptor subunit KA1 revealed by Cre expression in YAC transgenic mice. Brain Research, 876(1-2), 55-61. doi:10.1016/S0006-8993(00)02599-3.

引用: https://hdl.handle.net/11858/00-001M-0000-0028-2D32-5

要旨

To determine the spatio-temporal expression in brain of the high-affinity kainate receptor subunit KA1, we generated transgenic mice expressing Cre recombinase from the KA1 gene on a chromosomally integrated 550 kb yeast artificial chromosome (YAC). Activity of the KA1 gene promoter during brain development was visualized by Cre immunohistochemistry, and by X-gal staining of b-galactosidase induced by Cre recombinase in double transgenic KA1-Cre/lacZ indicator mice. During early brain development, expression from the YAC-carried KA1-Cre transgene was observed in all major brain areas, predicting a function for KA1 in the developing central nervous system. In the adult brain, KA1-Cre transgene expression was restricted mainly to hippocampal CA3 pyramidal and dentate gyrus granule cells, an adult expression pattern characteristic for the endogenous KA1 alleles. KA1-Cre transgenic mice may help in elucidating the role of floxed genes ablated in vivo in KA1 expressing neurons