Calcium sensitivity of glutamate release in a calyx-type terminal

Bollmann, Johann H.; Sakmann, Bert; Borst, J. Gerard G.

doi:10.1126/science.289.5481.953

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Calcium sensitivity of glutamate release in a calyx-type terminal

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Bollmann, Johann H.
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;

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Sakmann, Bert
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;

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Borst, J. Gerard G.
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;

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http://www.sciencemag.org/cgi/reprint/289/5481/953
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http://dx.doi.org/10.1126/science.289.5481.953
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Citation

Bollmann, J. H., Sakmann, B., & Borst, J. G. G. (2000). Calcium sensitivity of glutamate release in a calyx-type terminal. Science, 289(5481), 953-957. doi:10.1126/science.289.5481.953.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0028-307D-4

Abstract

Synaptic efÞcacy critically depends on the presynaptic intracellular calcium concentration ([Ca21]i). We measured the calcium sensitivity of glutamate release in a rat auditory brainstem synapse by laser photolysis of caged calcium. A rise in [Ca21]i to 1 micromolar readily evoked release. An increase to .30 micromolar depleted the releasable vesicle pool in ,0.5 millisecond. A comparison with action potentialÐevoked release suggested that a brief increase of [Ca21]i to ;10 micromolar would be sufÞcient to reproduce the physiological release pattern. Thus, the calcium sensitivity of release at this synapse is high, and the distinction between phasic and delayed release is less pronounced than previously thought.