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Journal Article

Photoelectrochemical bioanalysis of guanosine monophosphate via the use of coupled enzymatic reactions at a CdS/ZnS quantum dot electrode.

MPS-Authors
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Khan,  N.
Research Group of Enzyme Biochemistry, MPI for biophysical chemistry, Max Planck Society;

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Konrad,  M.
Research Group of Enzyme Biochemistry, MPI for biophysical chemistry, Max Planck Society;

External Resource

http://onlinelibrary.wiley.com/doi/10.1002/smll.201501883/epdf
(Publisher version)

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Supplementary Material (public)

2183344_Suppl.pdf
(Supplementary material), 2MB

Citation

Sabir, N., Khan, N., Völkner, J., Widdascheck, F., del Pino, P., Witte, G., et al. (2015). Photoelectrochemical bioanalysis of guanosine monophosphate via the use of coupled enzymatic reactions at a CdS/ZnS quantum dot electrode. Small, 11(43), 5844-5850. doi:10.1002/smll.201501883.


Cite as: https://hdl.handle.net/11858/00-001M-0000-0028-465F-E
Abstract
A photo-electrochemical sensor for the specific detection of guanosine monophosphate (GMP) is demonstrated, based on three enzymes combined in a coupled reaction assay. The first reaction involves the adenosine triphosphate (ATP)-dependent conversion of GMP to guanosine diphosphate (GDP) by guanylate kinase, which warrants substrate specificity. The reaction products ADP and GDPare co-substrates for the enzymatic conversion of phosphoenolpyruvate to pyruvate in a second reaction mediated by pyruvate kinase. Pyruvate in turn is the co-substrate for lactate dehydrogenase that generates lactate via oxidation of nicotinamide adenine dinucleotide (reduced form) NADH to NAD+. This third enzymatic reaction is electrochemically detected. For this purpose a CdS/ZnS quantum dot (QD) electrode is illuminated and the photocurrent response under fixed potential conditions is evaluated. The sequential enzyme reactions are first evaluated in solution. Subsequently, a sensor for GMP is constructed using polyelectrolytes for enzyme immobilization.