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Binding to Rab3A-interacting molecule RIM regulates the presynaptic recruitment of Munc13-1 and ubMunc13-2

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Andrews-Zwilling,  Yaisa S.
Molecular neurobiology, Max Planck Institute of Experimental Medicine, Max Planck Society;

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Kawabe,  Hiroshi
Molecular neurobiology, Max Planck Institute of Experimental Medicine, Max Planck Society;

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Reim,  Kerstin
Molecular neurobiology, Max Planck Institute of Experimental Medicine, Max Planck Society;

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Varoqueaux,  Frédérique
Molecular neurobiology, Max Planck Institute of Experimental Medicine, Max Planck Society;

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Brose,  Nils
Molecular neurobiology, Max Planck Institute of Experimental Medicine, Max Planck Society;

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Citation

Andrews-Zwilling, Y. S., Kawabe, H., Reim, K., Varoqueaux, F., & Brose, N. (2006). Binding to Rab3A-interacting molecule RIM regulates the presynaptic recruitment of Munc13-1 and ubMunc13-2. Journal of Biological Chemistry, 281(28), 19720-19731.


Cite as: http://hdl.handle.net/11858/00-001M-0000-002A-2507-9
Abstract
Transmitter release at synapses between nerve cells is spatially restricted to active zones, where synaptic vesicle docking, priming, and Ca2+-dependent fusion take place in a temporally highly coordinated manner. Munc13s are essential for priming synaptic vesicles to a fusion competent state, and their specific active zone localization contributes to the active zone restriction of transmitter release and the speed of excitation-secretion coupling. However, the molecular mechanism of the active zone recruitment of Munc13s is not known. We show here that the active zone recruitment of Munc13 isoforms Munc13-1 and ubMunc13-2 is regulated by their binding to the Rab3A-interacting molecule RIM1 alpha, a key determinant of long term potentiation of synaptic transmission at mossy fiber synapses in the hippocampus. We identify a single point mutation in Munc13-1 and ubMunc13-2 (I121N) that, depending on the type of assay used, strongly perturbs or abolishes RIM1 alpha binding in vitro and in cultured fibroblasts, and we demonstrate that RIM1 alpha binding-deficient ubMunc13-2(I121) is not efficiently recruited to synapses. Moreover, the levels of Munc13-1 and ubMunc13-2 levels are decreased in RIM1 alpha-deficient brain, and Munc13-1 is not properly enriched at active zones of mossy fiber terminals of the mouse hippocampus if RIM1 alpha is absent. We conclude that one function of the Munc13/RIM1 alpha interaction is the active zone recruitment of Munc13-1 and ubMunc13-2.