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A novel Conus peptide ligand for K+ channels

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Ferber,  Michael
Molecular and cellular neuropharmacology, Max Planck Institute of Experimental Medicine, Max Planck Society;

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Sporning,  Annett
Molecular and cellular neuropharmacology, Max Planck Institute of Experimental Medicine, Max Planck Society;

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Terlau,  Heinrich
Molecular and cellular neuropharmacology, Max Planck Institute of Experimental Medicine, Max Planck Society;

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Ferber, M., Sporning, A., Jeserich, G., DeLaCruz, R., Watkins, M., Olivera, B. M., et al. (2003). A novel Conus peptide ligand for K+ channels. The Journal of Biological Chemistry, 278(4), 2177-2183.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0029-2704-0
Abstract
Voltage-gated ion channels determine the membrane excitability of cells. Although many Conus peptides that interact with voltage-gated Na+ and Ca2+ channels have been characterized, relatively few have been identified that interact with K+ channels. We describe a novel Conus peptide that interacts with the Shaker K+ channel, M-kappa-conotoxin RIIIK from Conus radiatus. The peptide was chemically synthesized. Although M- kappa-conotoxin RIIIK is structurally similar to the mu- conotoxins that are sodium channel blockers, it does not affect any of the sodium channels tested, but blocks Shaker K+ channels. Studies using Shaker K+ channel mutants with single residue substitutions reveal that the peptide interacts with the pore region of the channel. Introduction of a negative charge at residue 427 (K427D) greatly increases the affinity of the toxin, whereas the substitutions at two other residues, Phe(425) and Thr (449), drastically reduced toxin affinity. Based on the Shaker results, a teleost homolog of the Shaker K+ channel, TSha1 was identified as a M-kappa-conotoxin RIIIK target. Binding of M-kappa-conotoxin RIIIK is state-dependent, with an IC50 of 20 nM for the closed state and 60 nm at 0 mV for the open state of TSha1 channels.