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Endothelin B receptor deficiency augments neuronal damage upon exposure to hypoxia-ischemia in vivo

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Sirén,  Anna Leena
Max Planck Institute of Experimental Medicine, Max Planck Society;

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Lewczuk,  Pjotr
Clinical neuroscience, Max Planck Institute of Experimental Medicine, Max Planck Society;

Hasselblatt,  M.
Max Planck Institute of Experimental Medicine, Max Planck Society;

Dembowski,  C.
Max Planck Institute of Experimental Medicine, Max Planck Society;

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Ehrenreich,  Hannelore
Clinical neuroscience, Max Planck Institute of Experimental Medicine, Max Planck Society;

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Citation

Sirén, A. L., Lewczuk, P., Hasselblatt, M., Dembowski, C., Schilling, L., & Ehrenreich, H. (2002). Endothelin B receptor deficiency augments neuronal damage upon exposure to hypoxia-ischemia in vivo. Brain Research, 945(1), 144-149.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0029-18EB-0
Abstract
The role of functional endothelin-B (ETB)-receptors on neuronal survival upon hypoxia-ischemia (HI) has been investigated in 14-day-old ETB-receptor-deficient spotting lethal (sl/sl) and wildtype (+/+) rats. Carotid ligation followed by exposure to 8% oxygen for 2 h produced distinct cortical and hippocampal neuronal damage. Damage severity 24 h after HI was mild to intermediate in +/+ rats whereas large cortical infarcts and profound apoptosis of the hippocampus evolved in sl/sl rats. The number of apoptotic cells in the dentate 24 h after HI amounted to 30+/-7 cells/0.1 mm(2) in sl/sl compared to 9+/- 3cells/0.1 mm(2) in wildtype rats (mean+/-S.E.M., n=10-11, P=0.0093). In-vitro hypoxia (15 h) resulted in a comparable increase in cell death in primary pure neuronal hippocampal cultures from both groups (49.8+/-1.6% in sl/sl, 51.4+/-0.9% in +/+, mean+/-S.E.M., n=5, P=0.0560). To conclude, absence of functional ETB receptors is associated with an increased susceptibility to HI in-vivo, which is not intrinsic to neurons. Antagonism of ETB receptors seems not to be desirable in ischemic stroke. (C) 2002 Elsevier Science B.V. All rights reserved.