English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

MTERF2 is a nucleoid component in mammalian mitochondria

MPS-Authors

Pellegrini,  Mina
Max Planck Society;

Asin-Cayuela,  Jorge
Max Planck Society;

Erdjument-Bromage,  Hediye
Max Planck Society;

Tempst,  Paul
Max Planck Society;

Larsson,  Nils-Goran
Max Planck Society;

Gustafsson,  Claes M
Max Planck Society;

Fulltext (public)
There are no public fulltexts available
Supplementary Material (public)
There is no public supplementary material available
Citation

Pellegrini, M., Asin-Cayuela, J., Erdjument-Bromage, H., Tempst, P., Larsson, N.-G., & Gustafsson, C. M. (2009). MTERF2 is a nucleoid component in mammalian mitochondria. Biochim Biophys Acta, 1787(5), 296-302.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0028-598A-F
Abstract
The mammalian MTERF family of proteins has four members, named MTERF1 to MTERF4, which were identified in homology searches using the mitochondrial transcription termination factor, mTERF (here denoted MTERF1) as query. MTERF1 and MTERF3 are known to participate in the control of mitochondrial DNA transcription, but the function of the other two proteins is not known. We here investigate the structure and function of MTERF2. Protein import experiments using isolated organelles confirm that MTERF2 is a mitochondrial protein. Edman degradation of MTERF2 isolated from stably transfected HeLa cells demonstrates that mature MTERF2 lacks a targeting peptide (amino acids 1-35) present in the precursor form of the protein. MTERF2 is a monomer in isolation and displays a non sequence-specific DNA-binding activity. In vivo quantification experiments demonstrate that MTERF2 is relatively abundant, with one monomer present per approximately 265 bp of mtDNA. In comparison, the mtDNA packaging factor TFAM is present at a ratio of one molecule per approximately 10-12 bp of mtDNA. Using formaldehyde cross-linking we demonstrate that MTERF2 is present in nucleoids, and therefore must be located in close proximity to mtDNA. Taken together, our work provides a basic biochemical characterization of MTERF2, paving the way for future functional studies.