日本語
 
Help Privacy Policy ポリシー/免責事項
  詳細検索ブラウズ

アイテム詳細


公開

学術論文

Cytoskeleton remodelling of confluent epithelial cells cultured on porous substrates

MPS-Authors
/persons/resource/persons182769

Steltenkamp,  S.
Micro Systems Technology, Center of Advanced European Studies and Research (caesar), Max Planck Society;

External Resource
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
フルテキスト (公開)
公開されているフルテキストはありません
付随資料 (公開)
There is no public supplementary material available
引用

Rother, J., Buchsenschutz-Gobeler, M., Noding, H., Steltenkamp, S., Samwer, K., & Janshoff, A. (2015). Cytoskeleton remodelling of confluent epithelial cells cultured on porous substrates. Journal of the Royal Society, Interface / the Royal Society, 12(103):. doi:10.1098/rsif.2014.1057.


引用: https://hdl.handle.net/11858/00-001M-0000-0028-62D4-2
要旨
The impact of substrate topography on the morphological and mechanical properties of confluent MDCK-II cells cultured on porous substrates was scrutinized by means of various imaging techniques as well as atomic force microscopy comprising force volume and microrheology measurements. Regardless of the pore size, ranging from 450 to 5500 nm in diameter, cells were able to span the pores. They did not crawl into the holes or grow around the pores. Generally, we found that cells cultured on non-porous surfaces are stiffer, i.e. cortical tension rises from 0.1 to 0.3 mN m(-1), and less fluid than cells grown over pores. The mechanical data are corroborated by electron microscopy imaging showing more cytoskeletal filaments on flat samples in comparison to porous ones. By contrast, cellular compliance increases with pore size and cells display a more fluid-like behaviour on larger pores. Interestingly, cells on pores larger than 3500 nm produce thick actin bundles that bridge the pores and thereby strengthen the contact zone of the cells.