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Journal Article

Microfluidic cryofixation for correlative microscopy

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Feindt,  H.
Micro Systems Technology, Center of Advanced European Studies and Research (caesar), Max Planck Society;

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Steltenkamp,  S.
Micro Systems Technology, Center of Advanced European Studies and Research (caesar), Max Planck Society;

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Citation

Mejia, Y. X., Feindt, H., Zhang, D., Steltenkamp, S., & Burg, T. P. (2014). Microfluidic cryofixation for correlative microscopy. Lab on a Chip, [Epub ahead of print]. doi:10.1039/C4LC00333K.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0028-62DD-0
Abstract
Cryofixation yields outstanding ultrastructural preservation of cells for electron microscopy, but current methods disrupt live cell imaging. Here we demonstrate a microfluidic approach that enables cryofixation to be performed directly in the light microscope with millisecond time resolution and at atmospheric pressure. This will provide a link between imaging/stimulation of live cells and post-fixation optical, electron, or X-ray microscopy.