Microfluidic cryofixation for correlative microscopy

Mejia, Y. X.; Feindt, H.; Zhang, D.; Steltenkamp, S.; Burg, T. P.

doi:10.1039/C4LC00333K

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Microfluidic cryofixation for correlative microscopy

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Feindt, H.
Micro Systems Technology, Center of Advanced European Studies and Research (caesar), Max Planck Society;

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Steltenkamp, S.
Micro Systems Technology, Center of Advanced European Studies and Research (caesar), Max Planck Society;

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http://dx.doi.org/10.1039/C4LC00333K
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http://pubs.rsc.org/en/Content/ArticleLanding/2014/LC/C4LC00333K
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Citation

Mejia, Y. X., Feindt, H., Zhang, D., Steltenkamp, S., & Burg, T. P. (2014). Microfluidic cryofixation for correlative microscopy. Lab on a Chip, [Epub ahead of print]. doi:10.1039/C4LC00333K.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0028-62DD-0

Abstract

Cryofixation yields outstanding ultrastructural preservation of cells for electron microscopy, but current methods disrupt live cell imaging. Here we demonstrate a microfluidic approach that enables cryofixation to be performed directly in the light microscope with millisecond time resolution and at atmospheric pressure. This will provide a link between imaging/stimulation of live cells and post-fixation optical, electron, or X-ray microscopy.