Fast determination of hydroxylated polychlorinated biphenyls in human plasma by 
online solid phase extraction coupled to liquid chromatography-tandem mass 
spectrometry.

Quinete, N.; Kraus, T.; Belov, V. N.; Aretz, C.; Esser, A.; Schettgen, T.

doi:10.1016/j.aca.2015.06.041

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Fast determination of hydroxylated polychlorinated biphenyls in human plasma by online solid phase extraction coupled to liquid chromatography-tandem mass spectrometry.

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Belov, V. N.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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http://www.sciencedirect.com/science/article/pii/S0003267015008429/pdfft?md5=c3db434e4c5d7f310d0180bfe3c43e64&pid=1-s2.0-S0003267015008429-main.pdf
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引用

Quinete, N., Kraus, T., Belov, V. N., Aretz, C., Esser, A., & Schettgen, T. (2015). Fast determination of hydroxylated polychlorinated biphenyls in human plasma by online solid phase extraction coupled to liquid chromatography-tandem mass spectrometry. Analytica Chimica Acta, 888, 94-102. doi:10.1016/j.aca.2015.06.041.

引用: https://hdl.handle.net/11858/00-001M-0000-0028-5B2F-D

要旨

Hydroxylated polychlorinated biphenyls (OH-PCBs) have been shown to be strongly retained in human blood causing endocrine-related toxicity, particularly on the thyroid system. Traditionally, analytical methods for the determination of OH-PCBs require labor-intensive and long-time consuming sample preparation with several extraction, evaporation and cleanup procedures steps and, in some cases, derivatization prior to the analysis by gas or liquid chromatography-mass spectrometry (GC-MS or LC-MS). The present study developed and validated a novel, sensitive and high throughput online solid phase extraction (SPE) method coupled to LC-tandem mass spectrometry (MS/MS) for the separation and quantitation of relevant congeners of OH-PCBs in human plasma. The developed method presented limits of quantification (LOQ) ranging from 0.02 to 0.5 ng mL(-1) and extraction recoveries from 71 to 134% for all congeners, requiring small amount of sample (only 100 mu L) and minimal sample preparation. In order to evaluate the applicability of the method, preliminary tests (N = 93) were conducted in plasma from individuals occupationally exposed to very high levels of PCBs in a German cohort. Penta-through hepta-chlorinated OH-PCBs were the predominant congeners in human plasma with concentrations up to 44.5 ng mL(-1), while lower chlorinated OH-PCBs were occasionally detected. In addition, a new PCB 28 metabolite has been synthesized and identified for the first time in human plasma and associations between OH-PCBs and their parent compounds in the studied cohort were also assessed.