English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Single-spike detection in vitro and in vivo with a genetic Ca2+ sensor

MPS-Authors
There are no MPG-Authors available
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Wallace, D. J., Meyer zum Alten Borgloh, S., Astori, S., Yang, Y., Bausen, M., Kugler, S., et al. (2008). Single-spike detection in vitro and in vivo with a genetic Ca2+ sensor. Nature Methods, 5(9), 797-804. doi:10.1038/nmeth.1242.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0028-64BA-E
Abstract
Measurement of population activity with single-action-potential, single-neuron resolution is pivotal for understanding information representation and processing in the brain and how the brain's responses are altered by experience. Genetically encoded indicators of neuronal activity allow long-term, cell type-specific expression. Fluorescent Ca2+ indicator proteins (FCIPs), a main class of reporters of neural activity, initially suffered, in particular, from an inability to report single action potentials in vivo. Although suboptimal Ca2+-binding dynamics and Ca2+-induced fluorescence changes in FCIPs are important factors, low levels of expression also seem to play a role. Here we report that delivering D3cpv, an improved fluorescent resonance energy transfer-based FCIP, using a recombinant adeno-associated virus results in expression sufficient to detect the Ca2+ transients that accompany single action potentials. In upper-layer cortical neurons, we were able to detect transients associated with single action potentials firing at rates of <1 Hz, with high reliability, from in vivo recordings in living mice.