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Journal Article

SiR–Hoechst is a far-red DNA stain for live-cell nanoscopy.

MPS-Authors
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Lukinavicius,  G.
Laboratory of Chromatin Labeling and Imaging, Max Planck Institute for Biophysical Chemistry, Max Planck Society;

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D'Este,  E.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Hell,  S. W.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

Fulltext (public)

2227350.pdf
(Publisher version), 3MB

Supplementary Material (public)

2227350_Suppl_1.pdf
(Supplementary material), 444KB

2227350_Suppl_2.mov
(Supplementary material), 2MB

2227350_Suppl_3.mov
(Supplementary material), 4MB

Citation

Lukinavicius, G., Blaukopf, C., Pershagen, E., Schena, A., Reymond, L., Derivery, E., et al. (2015). SiR–Hoechst is a far-red DNA stain for live-cell nanoscopy. Nature Communications, 6: 8497. doi:10.1038/ncomms9497.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0029-04AF-4
Abstract
Cell-permeable DNA stains are popular markers in live-cell imaging. Currently used DNA stains for live-cell imaging are either toxic, require illumination with blue light or are not compatible with super-resolution microscopy, thereby limiting their utility. Here we describe a far-red DNA stain, SiR–Hoechst, which displays minimal toxicity, is applicable in different cell types and tissues, and is compatible with super-resolution microscopy. The combination of these properties makes this probe a powerful tool for live-cell imaging.