Real-time label-free direct electronic monitoring of topoisomerase enzyme 
binding kinetics on graphene.

Zuccaro, L.; Tesauro, C.; Kurkina, T.; Fiorani, P.; Yu, H. K.; Knudsen, B. R.; Kern, K.; Desideri, A.; Balasubramanian, K.

doi:10.1021/acsnano.5b05709

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Real-time label-free direct electronic monitoring of topoisomerase enzyme binding kinetics on graphene.

MPS-Authors

/persons/resource/persons131049

Yu, H. K.
Department of Dynamics at Surfaces, MPI for Biophysical Chemistry, Max Planck Society;

External Resource

http://pubs.acs.org/doi/pdf/10.1021/acsnano.5b05709
(Publisher version)

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Zuccaro, L., Tesauro, C., Kurkina, T., Fiorani, P., Yu, H. K., Knudsen, B. R., et al. (2015). Real-time label-free direct electronic monitoring of topoisomerase enzyme binding kinetics on graphene. ACS Nano, 9(11), 11166-11176. doi:10.1021/acsnano.5b05709.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0029-3312-D

Abstract

Monolayer graphene field-effect sensors operating in liquid have been widely deployed for detecting a range of analyte species often under equilibrium conditions. Here we report on the real-time detection of the binding kinetics of the essential human enzyme, topoisomerase I interacting with substrate molecules (DNA probes) that are immobilized electrochemically on to monolayer graphene strips. By monitoring the field-effect characteristics of the graphene biosensor in real-time during the enzyme–substrate interactions, we are able to decipher the surface binding constant for the cleavage reaction step of topoisomerase I activity in a label-free manner. Moreover, an appropriate design of the capture probes allows us to distinctly follow the cleavage step of topoisomerase I functioning in real-time down to picomolar concentrations. The presented results are promising for future rapid screening of drugs that are being evaluated for regulating enzyme activity.