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Morpholgy and dynamcis of the endocytic pathway in Dictyostelium discoideum

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Neuhaus,  Eva Maria
Department of Molecular Cell Research, Max Planck Institute for Medical Research, Max Planck Society;

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Almers,  Wolfhard
Department of Molecular Cell Research, Max Planck Institute for Medical Research, Max Planck Society;

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Soldati,  Thierry
Department of Molecular Cell Research, Max Planck Institute for Medical Research, Max Planck Society;

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Citation

Neuhaus, E. M., Almers, W., & Soldati, T. (2002). Morpholgy and dynamcis of the endocytic pathway in Dictyostelium discoideum. Molecular and Cellular Biology (Washington, DC), 13(4), 1390-1407. doi:10.1091/mbc.01-08-0392.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0029-75C6-0
Abstract
Dictyostelium discoideum is a genetically and biochemically tractable social amoeba belonging to the crown group of eukaryotes. It performs some of the tasks characteristic of a leukocyte such as chemotactic motility, macropinocytosis, and phagocytosis that are not performed by other model organisms or are difficult to study.D. discoideum is becoming a popular system to study molecular mechanisms of endocytosis, but the morphological characterization of the organelles along this pathway and the comparison with equivalent and/or different organelles in animal cells and yeasts were lagging. Herein, we used a combination of evanescent wave microscopy and electron microscopy of rapidly frozen samples to visualize primary endocytic vesicles, vesicular-tubular structures of the early and late endo-lysosomal system, such as multivesicular bodies, and the specialized secretory lysosomes. In addition, we present biochemical and morphological evidence for the existence of a micropinocytic pathway, which contributes to the uptake of membrane along side macropinocytosis, which is the major fluid phase uptake process. This complex endosomal compartment underwent continuous cycles of tubulation/vesiculation as well as homo- and heterotypic fusions, in a way reminiscent of mechanisms and structures documented in leukocytes. Finally, egestion of fluid phase from the secretory lysosomes was directly observed.